Part:BBa_K942004
Shuttle Sequence (SS+B.P.S.)+Der f 2+His
Der f 2 is a major allergen from Dermatophagoides farinae, although its biological function in the dust mite has not been well discovered yet. It comes flanked with the α-mating factor pre-sequence for protein secretion.
This protein has an estimated weight of 16.6kDa. It has a folding similar to that of an immunoglobulin and also has a suspected antibacterial activity, yet it has been expressed previously in E.coli. We recommend its affinity purification using the 6x His tag. (Liu Z, 2007)
About Safety
The Der f 2 protein is a major allergen from the dust mite, so any contact with cells transformed with this part should be done with appropriate gloves. Also we recommend that the use must make sure he/she is not allergic to dust mite. The secretion of this protein makes this part more hazardous so you would like to have extra precaution to prevent any leak from your samples.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 573
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